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Quantitative Human HYOU1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX956)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human HYOU1 (glycosylated) sandwich ELISA kit is designed to detect human hypoxia up regulated 1 (HYOU1) levels. HYOU1 is a molecular chaperone, which is located in the endoplasmic reticulum (ER). The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.083 ng/mL, which can accurately detect low concentrations of HYOU1 in the sample.
Target HYOU1
N-Glycosylation Site 155, 222, 515, 596, 830, 862, 869, 922, 931
Sample Types Tissue homogenates, serum, plasma
Sample Volume 100 μL
Sensitivity 0.083 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Hypoxia up regulated 1
Alternate Names HYOU1; Hypoxia up regulated 1; ORP150; Glucose-regulated protein 170; 150 kDa oxygen-regulated protein
Uniprot No. Q9Y4L1
Application The quantitative human HYOU1 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated HYOU1 levels in biological samples. The assay is valuable for investigating the role of HYOU1 in protein folding, ER stress, and cellular adaptation to hypoxia.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 85-105%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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