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Quantitative Human NOX4 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX834)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human NOX4 (glycosylated) sandwich ELISA kit is designed to detect human nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) levels. NOX4 is an enzyme that is a member of the NADPH oxidase family. NOX4 generates reactive oxygen species (ROS). The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.092 ng/mL, which can accurately detect low concentrations of NOX4 in the sample.
Target NOX4
N-Glycosylation Site 133, 230
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.092 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Nicotinamide adenine dinucleotide phosphate oxidase 4
Alternate Names NOX4; Nicotinamide adenine dinucleotide phosphate oxidase 4; KOX1; NADPH oxidase 4
Uniprot No. Q9NPH5
Application The quantitative human NOX4 (glycosylated) sandwich ELISA kit is used to measure NOX4 levels in biological samples. This kit is applicable in studies examining oxidative stress and cardiovascular diseases. It is also relevant to studies investigating the role of NOX4 in cellular signaling pathways and disease pathogenesis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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