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Quantitative Human PDCN (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX831)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PDCN (glycosylated) sandwich ELISA kit is designed to detect human podocin (PDCN) levels. PDCN is a small leucine-rich proteoglycan that plays a role in regulating cell adhesion and matrix assembly. The kit is suitable for various biological samples such as tissue homogenates, cell lysates, urine. Its sensitivity is 0.415 ng/mL, which can accurately detect low concentrations of PDCN in the sample.
Target PDCN
N-Glycosylation Site 287
Sample Types Tissue homogenates, cell lysates, urine
Sample Volume 100 μL
Sensitivity 0.415 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1.25 ng/mL-40 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Podocin
Alternate Names PDCN; Podocin; NPHS2; Nephrosis 2
Uniprot No. Q9NP85
Application The quantitative human PDCN (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of PDCN in biological samples. This kit is applicable in studies examining cell-matrix interactions and kidney diseases. It is also relevant to studies investigating the role of PDCN in tissue remodeling and fibrosis.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 90-105%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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