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Quantitative Human PKBγ (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX908)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human PKBγ (glycosylated) sandwich ELISA kit is designed to detect human protein kinase Bγ (PKBγ) levels. PKBγ is a serine/threonine protein kinase. PKBγ is a member of the Akt family of kinases, which play a critical role in cellular signaling pathways involved in cell growth, proliferation, and survival. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.837 ng/mL, which can accurately detect low concentrations of PKBγ in the sample.
Target PKBγ
O-Glycosylation Site 302, 309, 472
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.837 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 2.50 ng/mL-85 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Protein kinase Bγ
Alternate Names PKBγ; Protein kinase Bγ; PKBG; RAC-PK-gamma; V-Akt murine thymoma viral oncogene homolog 3
Uniprot No. Q9Y243
Application The quantitative human PKBγ (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated PKBγ levels in biological samples. The assay is valuable for investigating the role of PKBγ in cancer, neurological disorders, and metabolic diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-90%;
Plasma sample: n=4, 85-100%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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