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Quantitative Human SEMA4B (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX835)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human SEMA4B (glycosylated) sandwich ELISA kit is designed to detect human semaphorin 4B (SEMA4B) levels. SEMA4B is a transmembrane protein that belongs to the semaphorin family. The kit is suitable for various biological samples such as tissue homogenates. Its sensitivity is 0.082 ng/mL, which can accurately detect low concentrations of SEMA4B in the sample.
Target SEMA4B
N-Glycosylation Site 69, 96, 165, 410, 525, 630
Sample Types Tissue homogenates
Sample Volume 100 μL
Sensitivity 0.082 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 1 ng/mL-10 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name Semaphorin 4B
Alternate Names SEMA4B; Semaphorin 4B; SEMAC
Uniprot No. Q9NPR2
Application The quantitative human SEMA4B (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of SEMA4B in biological samples. This kit is useful in studies examining axon guidance and neuronal development. It is also relevant to studies investigating the role of SEMA4B in immune regulation and tumor biology.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 85-105%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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