Quantitative Human SIAE (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX820)

Name: Quantitative Human SIAE (Glycosylated) Sandwich ELISA Kit
SKU: GLJF-0525-HX820
Rating: 5 (1 reviews)

Datasheet

Size	Qty	Add To Basket
1×48 T
1×96 T

Product Description	The quantitative human SIAE (glycosylated) sandwich ELISA kit is designed to detect human sialic acid acetylesterase (SIAE) levels. SIAE is an enzyme that catalyzes the hydrolysis of sialic acid esters. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.086 ng/mL, which can accurately detect low concentrations of SIAE in the sample.
Target	SIAE
N-Glycosylation Site	107, 138, 267, 290, 401, 422
Sample Types	Tissue homogenates, cell lysates
Sample Volume	100 μL
Sensitivity	0.086 ng/mL
Detection Principle	Quantitative sandwich ELISA
Detection Range	1 ng/mL-10 ng/mL
Detection Time	1 h-5 h
Detection Wavelength	450 nm
Storage	Store at 2-8°C for long term storage.

Species	Human
Full Name	Sialic acid acetylesterase
Alternate Names	SIAE; Sialic acid acetylesterase; CSEC; LSE; Sialic acid-specific acetylesterase II
Uniprot No.	Q9HAT2

Application	The quantitative human SIAE (glycosylated) sandwich ELISA kit is used to determine SIAE levels in biological samples. This kit is applicable in studies examining sialic acid metabolism and cell surface modifications. It is also relevant to studies investigating the role of sialic acids in immune recognition and disease.
Kit Components	Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision	Intra-Assay: n=20, CV <8%; Inter-Assay: n=20, CV <10%;
Recovery	Serum sample: n=5, 90-105%; Plasma sample: n=4, 80-95%;
Standard Curve	The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.

For Research Use Only.

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