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Quantitative Human SIGLEC7 (Glycosylated) Sandwich ELISA Kit

CAT#: GLJF-0525-HX910

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Specifications
Product Description
The quantitative human SIGLEC7 (glycosylated) sandwich ELISA kit is designed to detect human sialic acid binding Ig like lectin 7 (SIGLEC7) levels. SIGLEC7 is a transmembrane protein that belongs to the Siglec family. Siglecs are a group of proteins that bind to sialic acids, which are sugar molecules found on the surface of cells. The kit is suitable for various biological samples such as tissue homogenates, serum, plasma. Its sensitivity is 0.097 ng/mL, which can accurately detect low concentrations of SIGLEC7 in the sample.
Target
SIGLEC7
Kit Components
Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Sample Types
Tissue homogenates, serum, plasma
Sample Volume
100 μL
Sensitivity
0.097 ng/mL
Detection Principle
Quantitative sandwich ELISA
Detection Range
1 ng/mL-10 ng/mL
Detection Time
1 h-5 h
Detection Wavelength
450 nm
Storage
Store at 2-8°C for long term storage.
Target Information
Species
Human
Full Name
Sialic acid binding Ig like lectin 7
Alternate Names
SIGLEC7; Sialic acid binding Ig like lectin 7; CD328; AIRM1; QA79; Adhesion inhibitory receptor molecule 1; QA79 membrane protein
Uniprot No.
N-Glycosylation Site
105, 142, 165, 229, 235, 242, 260, 334
Application Notes
Application
The quantitative human SIGLEC7 (glycosylated) sandwich ELISA kit is designed for the quantitative measurement of glycosylated SIGLEC7 levels in biological samples. The assay is useful for investigating the role of SIGLEC7 in immune regulation, cancer, and inflammatory diseases.
Precision
Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery
Serum sample: n=5, 85-100%;
Plasma sample: n=4, 85-105%;
Standard Curve

The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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