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Quantitative Human UGGT1 (Glycosylated) Sandwich ELISA Kit (CAT#: GLJF-0525-HX858)

Datasheet
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1×48 T
1×96 T

Product Description The quantitative human UGGT1 (glycosylated) sandwich ELISA kit is designed to detect human UDP-glucose glycoprotein glucosyltransferase 1 (UGGT1) levels. UGGT1 is an enzyme that is located in the endoplasmic reticulum. UGGT1 plays a crucial role in protein folding. This enzyme monitors the folding status of newly synthesized glycoproteins. The kit is suitable for various biological samples such as tissue homogenates, cell lysates. Its sensitivity is 0.128 ng/mL, which can accurately detect low concentrations of UGGT1 in the sample.
Target UGGT1
N-Glycosylation Site 536, 1228
Sample Types Tissue homogenates, cell lysates
Sample Volume 100 μL
Sensitivity 0.128 ng/mL
Detection Principle Quantitative sandwich ELISA
Detection Range 0.55 ng/mL-15 ng/mL
Detection Time 1 h-5 h
Detection Wavelength 450 nm
Storage Store at 2-8°C for long term storage.
Species Human
Full Name UDP-glucose glycoprotein glucosyltransferase 1
Alternate Names UGGT1; UDP-glucose glycoprotein glucosyltransferase 1; UGTR; UGCGL1; UDP-glucose ceramide glucosyltransferase-like 1
Uniprot No. Q9NYU2
Application The quantitative human UGGT1 (glycosylated) sandwich ELISA kit is used to measure UGGT1 levels in biological samples. This kit is useful in studies examining protein folding and endoplasmic reticulum stress. It is also relevant to studies investigating the role of UGGT1 in quality control mechanisms within the cell and its involvement in various protein folding diseases.
Kit Components Pre-coated ELISA plate; Lyophilized standard; Biotin-labeled antibody; HRP-avidin; Various diluents; Wash buffer; TMB chromogenic substrate; Stop solution
Precision Intra-Assay: n=20, CV <8%;
Inter-Assay: n=20, CV <10%;
Recovery Serum sample: n=5, 80-95%;
Plasma sample: n=4, 80-95%;
Standard Curve
The standard curve is for reference only, and a new standard curve should be generated for each set of samples tested.
For Research Use Only.
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