GlycoErase™ B3GNT8 Knockout Human Lung Cancer Cell A-427 (CAT#: GLJF-0525-JF1400)

Description	The B3GNT8 knockout A-427 cell line, deficient in UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 8 involved in exosomal poly-N-acetyllactosamine biosynthesis, enables the study of exosomal poly-N-acetyllactosamine disruption in lung adenocarcinoma, potentially affecting exosome glycosylation, exosome-mediated signaling, and the functions of exosomal glycans within the tumor microenvironment.
Product Type	KO Cell Lines
Species	Human
Cell Morphology	Epithelial-like, adherent
Passage Ratio	1:2~1:3
Cell Line	A-427
Primary Disease	Non-Small Cell Lung Cancer
Lineage	Lung

Cell Viability	>90%
Sterility Test	The sterility test indicated an absence of microbial growth.
Identity Test	STR identification
Mycoplasma Test	Negative
Virus Test	Negative for HIV, HBV and HCV.
Genetic Stability Testing	We conduct cell genetic stability studies in full compliance with ICH guidelines. Our expertise enables us to design and execute a comprehensive testing program tailored to your specific needs and regulatory requirements.
Validation	PCR, Sanger Sequencing
Culture Medium	FBS & RPMI
Application	Functional assay
Size	1 M cells/vial*2
Product Format	Frozen
Shipping	Dry ice
Availability Status	Made to order
Handling Notes	Upon receipt, this product must be immediately transferred from dry ice to liquid nitrogen (-150°C to -190°C) and stored in a liquid nitrogen tank. Cell viability is critically dependent on proper handling. We cannot guarantee viability if these instructions are not strictly adhered to.
Product Disclaimer	This product is provided for research only, not suitable for human or animal use. Due to the inherent limitations of infectious agent testing, investigators must exercise extreme caution when handling cells provided by Creative Biolabs, treating all cells as potentially biohazardous.

Target	B3GNT8
Full Name	UDP-GlcNAc:BetaGal Beta-1,3-O-acetylglucosaminyltransferase 8
Alternative Name	beta3Gn-T8, B3GALT7, BGALT15
Location	19q13.2
Gene ID	374907
Summary	Protein O-acetylglucosaminyltransferase involved in poly-N-acetyllactosamine biosynthesis; located in extracellular exosomes.