Caco-2 In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

This assay is to provide Caco-2-based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Caco-2

Human

Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.

Caco2

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

Caco-2-cell based In Vitro 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay (Oxidative Stress)

It's a commonly used simple antioxidant assay. In the DPPH assays, DPPH free radical (DPPH) accepts protons from compounds with antioxidant properties to form yellow colored stable DPPH.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Antioxidant Assay