Intratumoral Microbiota Analysis Services by Multiple Modality Integrated Method

Intratumoral Microbiota: A Key to Cancer Progression

The tumor microenvironment is a complex ecosystem, increasingly recognized as harboring distinct microbial communities. Recent studies highlight that intratumoral bacteria significantly influence tumor progression, metastasis, and critically, response to cancer therapies, particularly immunotherapies. Understanding these intricate host-microbe interactions is essential for identifying novel therapeutic targets and biomarkers, overcoming treatment resistance, and advancing personalized oncology. The necessity to precisely characterize this hidden facet of cancer biology is paramount for next-generation drug development.

Fig.1 Intratumoral microbiome exert pro-tumor and anti-tumor effects through immune regulation. (OA Literature)

Fig.1 The intratumoral microbiota has both pro- and anti-tumor effects through immune modulation.¹

Multiple-modality Integrated Methods-based Intratumoral Microbiota Analysis Services at Creative Biolabs

Creative Biolabs' multiple-modality integrated methods-based intratumoral microbiota analysis services provide comprehensive solutions for deciphering the complex interplay between intratumoral microbes and cancer biology. This involves a meticulous process of sample preparation, multi-omic sequencing, and integrated bioinformatics analysis to generate actionable insights into microbial composition, spatial distribution, functional roles, and their impact on therapeutic responses. The typical timeframe for this detailed analysis ranges from 8 to 16 weeks, culminating in high-resolution data and expert interpretation that empowers your research and development efforts with identified microbial and host biomarkers. Our commitment to quality assurance is paramount, with rigorous QC checks at every stage, from nucleic acid extraction to data processing, ensuring the reliability and accuracy of your results.

Service Packages

To cater to diverse research needs, Creative Biolabs offers specialized service packages focused on intratumoral microbiota analysis:

Intratumoral Microbiota Single-Cell Analysis Service

This service provides high-resolution insights into microbial communities and host-microbe interactions at the single-cell level within the tumor microenvironment. It helps uncover spatial heterogeneity and cell-type specific associations that bulk analysis might miss.

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Intratumoral Microbiota Traceability Analysis Service

Utilizing advanced tracing techniques, this service helps pinpoint the origin and dynamics of intratumoral microbes, offering critical information for understanding how bacteria infiltrate and establish within tumor tissues.

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Comprehensive In Vivo Modeling Solution for Intratumoral Microbiota Dynamics Research

We offer robust in vivo models and experimental designs to study the impact of intratumoral microbiota on tumor growth, metastasis, and response to various therapies in a living system.

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Comprehensive In Vitro Modeling Solution for Intratumoral Microbiota Dynamics Research

Our in vitro services provide controlled experimental platforms to dissect specific host-microbe interactions, test therapeutic interventions, and explore the functional consequences of intratumoral microbes outside of a living organism.

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Workflow

Step 1: Sample Processing & Nucleic Acid Extraction

Initial tissue processing, followed by optimized protocols for high-yield, high-quality DNA and RNA extraction from both microbial and host components, minimizing contamination.

Step 2: Multi-omic Data Acquisition

This stage involves generating comprehensive datasets. We employ techniques such as 16S rRNA gene sequencing for bacterial community profiling, shotgun metagenomic sequencing for taxonomic and functional potential, metatranscriptomics for active microbial gene expression, and host transcriptome analysis (RNA-seq) for immune response and pathway alterations. For spatial context, advanced imaging and spatial transcriptomics may also be integrated.

Step 3: Advanced Data Pre-processing & Quality Control

Rigorous quality assessment and filtering of raw sequencing data, including host DNA/RNA removal from microbial datasets, to ensure data integrity and reliability for downstream analysis.

Step 4: Integrated Bioinformatics Analysis

Sophisticated computational pipelines are utilized to integrate diverse multi-omic datasets. This includes microbial taxonomic and phylogenetic analysis, functional pathway prediction, co-occurrence network analysis, and correlation of microbial features with host gene expression and clinical outcomes.

Step 5: Data Interpretation & Report Generation

Expert bioinformaticians and biologists interpret the integrated findings, identifying key microbial taxa, functional modules, and host immune pathways influenced by the intratumoral microbiome.

Our Service Highlights

Holistic Tumor-Microbe View: We combine genomic, transcriptomic, and spatial data for a complete understanding of the tumor-microbe ecosystem.
Enhanced Biomarker Identification: Our integrated approach significantly improves the precision in identifying novel and relevant biomarkers.
Novel Therapeutic Target Discovery: By unraveling complex interactions, we help uncover new therapeutic targets for more effective interventions.
Improved Treatment Response Prediction: Gain insights that can help predict patient response to various cancer treatments, especially immunotherapies.
Streamlined R&D: Our comprehensive data and expert interpretation accelerate your research and development pipeline.

FAQs

Q1: How does your multi-modality approach differ from standard 16S rRNA gene sequencing?

A1: While 16S rRNA gene sequencing identifies bacterial communities, our multi-modality approach integrates this with shotgun metagenomics (for functional potential and species-level resolution), metatranscriptomics (for active microbial gene expression), and host transcriptomics (for host-response insights). This holistic view provides a deeper understanding of microbial activity and interaction within the tumor.

Q2: Can your service help predict patient response to specific cancer therapies?

A2: By correlating microbial profiles and functional pathways with patient clinical data and treatment outcomes, our integrated analysis can help identify microbial signatures associated with response or resistance to various therapies, including immunotherapy. This information can inform future stratification and treatment optimization strategies.

Q3: How does this service compare to analyzing microbiota from other sites, like the gut?

A3: While gut microbiota research is well-established, intratumoral microbiota presents unique challenges and opportunities due to its direct interaction with cancer cells and the tumor microenvironment. Our specialized methods are optimized for low biomass samples and the complex cellular matrix of tumor tissues, providing targeted insights specific to the tumor itself, which are distinct from systemic or gut-based analyses.

Why Choose Creative Biolabs

Creative Biolabs' multiple-modality integrated methods-based intratumoral microbiota analysis services empower researchers to delve deeper into the complex relationship between intratumoral microbes and cancer. By providing comprehensive, high-resolution insights into the tumor microenvironment, we help accelerate biomarker discovery, therapeutic target identification, and ultimately, the development of more effective cancer treatments. If you are interested in our multiple-modality integrated methods-based intratumoral microbiota analysis services, please click the link to get in touch with us.

Reference

Wang, Na et al. "Intratumoral microbiome: implications for immune modulation and innovative therapeutic strategies in cancer." Journal of biomedical science vol. 32,1 23. 19 Feb. 2025. Distributed under Open Access License CC BY 4.0, without modification. DOI: https://doi.org/10.1186/s12929-025-01117-x