Cat IL-10 (IL10) ELISA Kit, Lot 21AO-470 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P381
Product Type: ELISA Kit
Target: IL-10 (IL10)
Short Description
The kit is designed for in vitro quantitative measurement of Cat IL-10 (IL10) in Cell Culture Supernatant, Plasma, Serum.
Description
Feline IL-10 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Applications
ELISA
Application Notes
Recommended Dilution for serum and plasma samples: 2-fold
Target
IL-10 (IL10)
Reactivity
Cat
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Cell Culture Supernatant, Plasma, Serum
Specificity
This ELISA antibody pair detects feline IL-10. Approximately 15 % cross-reactivity with recombinant canine and 1 % cross-reactivity with recombinant human IL-10 is observed.
Components
Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate
Material not included
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis
Sensitivity
0.2 ng/mL
Sample Volume
100 μL
Plate
Pre-coated
Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Note
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Restrictions
For Research Use only
Alternative Name
Interleukin 10 (IL-10)
Synonyms
CSIF; GVHDS; IL-10; IL10A; TGIF; Il-10; IL10X; interleukin 10; IL10; Il10
Background
Gene Names: IL10
Protein names: Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Gene ID
493683
UniProt
P55029
Pathways
Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints
Protocol
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.
For Research Use Only | Not For Clinical Use
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