Short Description

Quantifies skin membrane permeability of test compounds using the PAMPA method.

Brand

CelluTest

Development Stage

Research-Grade Cell-Based Assay Development

Description

CelluTest™ Parallel Artificial Membrane Permeability Assay (PAMPA)-Skin Kit provides a high-efficiency in vitro solution for quantifying the passive transdermal permeability of drug candidates and cosmetic active ingredients. By utilizing a sophisticated lipid-infused artificial membrane that mimics the complex barrier properties of the human stratum corneum, this system offers a robust alternative to limited biological skin tissues and low-throughput cell-based models.

Features

Biomimetic Fidelity: Utilizes a specialized lipid oil mixture to accurately simulate the human stratum corneum's unique barrier properties.
High-Throughput Compatibility: Optimized for 96-well formats, enabling rapid, parallel screening of diverse chemical libraries with minimal sample volume.
Robust Reproducibility: Engineered with standardized components to ensure consistent inter-assay results, facilitating precise quantitative rank-ordering of lead compounds.
Operational Simplicity: Streamlined protocol eliminates complex tissue handling, providing a cost-effective alternative to traditional animal or human skin models.

Process Relevance

Predicting passive percutaneous absorption to accelerate lead selection and optimize topical formulation strategies during early-stage development.

Application Stage

Lead optimization, ADME profiling, and topical formulation screening during preclinical drug discovery phases.

Applications

Evaluating passive diffusion of oncology candidates, dermatological drugs, and cosmetic actives through synthetic barriers to determine permeability coefficients and absorption kinetics.

Qualified With

Internal performance validation using reference standards under defined assay conditions.

Target

Passive transdermal diffusion rates of small molecule drug candidates and chemical compounds.

Detection Method

PAMPA

Sample Type

Chemical compounds

Research Areas

Transdermal drug delivery, pharmacokinetics (ADME), oncology therapeutics, dermatology, skin physiology, etc.

Size
96 tests

Data Output Type
Quantitative permeability coefficient calculated via endpoint spectrophotometric or fluorometric detection.

Assay Time
20 h

Species
All Species

Storage
Store at −20 °C; upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.

Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 ℃ storage after handling. Do not store at elevated temperatures for extended periods.

Expiry Date
12 months under recommended storage conditions to ensure optimal performance.

Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.

Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.

Shipping
Shipped at room temperature.

Background
The stratum corneum acts as a formidable rate-limiting barrier for systemic drug delivery. Understanding passive diffusion kinetics is vital for developing effective topical oncological treatments and managing drug bioavailability.

Pathways
This assay models the extracellular lipid matrix pathway within the skin. It focuses on the transcellular and intercellular movement of solutes, reflecting critical absorption mechanisms.

CelluTest™ Parallel Artificial Membrane Permeability Assay-Skin Kit (CAT#: ITS-1125-WB1)

Overview

Specification

Target Details