ELISA Kit for quantitative detection of mouse IL-5
Applications
ELISA
Qualified With
IL-5
Reactivity
Mouse
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Biological fluids such as serum, plasma, and cell culture supernatants.
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-5. No significant cross-reactivity or interference between IL-5 and analogues was observed. Note: Limited by current skills and knowledge, it is difficult for us to complete the cross-reactivity detection between IL-15 and all the analogues, therefore, cross reaction may still exist.
Components
Pre-coated, ready to use 96-well strip plate Plate sealer for 96 wells Standard Sample/Standard Dilution Buffer Biotin-labeled Antibody (Concentrated) Antibody Dilution Buffer HRP-Streptavidin Conjugate (SABC) SABC Dilution Buffer TMB Substrate Stop Solution Wash Buffer (25 x concentrate) Instruction manual
Material not included
1.Microplate reader (wavelength:450nm) 2.37 °C incubator 3.Automated plate washer 4.Precision single and multi-channel pipette and disposable tips 5.Clean tubes 6.Deionized or distilled water
Sensitivity
2 pg/mL
Sample Volume
100 μL
Plate
Pre-coated
Reagent Preparation
Bring all reagents and samples to room temperature for 20 minutes before use. Wash Buffer Standards Preparation of Biotin-labeled Antibody Working Solution Preparation of HRP-Streptavidin Conjugate (SABC) Working Solution
Assay Procedure
Set standard, test samples (diluted at least 1/2 with Sample Dilution Buffer), control (blank) wells on the pre-coated plate respectively, and then, records their positions.Wash plate 2 times before adding standard, sample and control (blank) wells! Prepare Standards Add Samples Incubate Wash Biotin-labeled Antibody Wash HRP-Streptavidin Conjugate (SABC) Wash TMB Substrate Stop OD Measurement
Assay Precision
Intra-Assay: CV<4.2% Inter-Assay: CV<7.8%
Storage
Store Standard at -20°C; Store other components at 2-8°C.
Expiry Date
Stable as supplied until expiry date on label.
Note
Tested for sterility
Restrictions
Restrictions for research use only. Intended for use by laboratory professionals.
This gene encodes a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils. The encoded cytokine plays a major role in the regulation of eosinophil formation, maturation, recruitment and survival. The increased production of this cytokine may be related to pathogenesis of eosinophil-dependent inflammatory diseases. This cytokine functions by binding to its receptor, which is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF). This gene is located on chromosome 5 within a cytokine gene cluster which includes interleukin 4 (IL4), interleukin 13 (IL13), and CSF2 . This gene, IL4, and IL13 may be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.
Gene ID
16191
UniProt
P04401
Pathways
Among its related pathways are Allograft rejection and PEDF Induced Signaling.
Protocol
1.Wash plate 2 times before adding Standard, Sample and Control wells! 2.Add 100 µL standard or sample to each well and incubate for 90 minutes at 37 °C. 3.Aspirate and wash plates 2 times. 4.Add 100 µL Biotin-labeled antibody working solution to each well and incubate for 60 minutes at 37 °C. 5.Aspirate and wash plates 3 times. 6.Add 100 µL SABC Working Solution into each well and incubate for 30 minutes at 37 °C. 7.Aspirate and wash plates 5 times. 8.Add 90 µL TMB Substrate Solution. Incubate 10-20 minutes at 37 °C. 9.Add 50 µL Stop Solution. Read at 450nm immediately and calculation.
