PharmaOnco™ HEK293-Tg(Human TLR4//MD2//CD14) Stable Cell Line, Cell Pool/Single Clone (CAT#: ITS-0822-HMM76)

Creative Biolabs offers HEK293-Tg(Human TLR4//MD2//CD14) stable cell line which the TLR4 and MD2 and CD14 gene stably expressed in HEK293 cells.

Creative Biolabs offers the overexpression HEK293 cells stably expressing additional TLR or NOD genes.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

TLR4 and MD2 and CD14

Human

HEK293

TLR4 is a receptor that detects lipopolysaccharides (LPS) from Gram-negative bacteria. It is crucial for initiating innate immune responses and activating pro-inflammatory cascades. TLR4 can also recognize other danger signals, contributing to the immune response to infections and tissue damage.
MD2 is a small glycoprotein that associates with Toll-like receptor 4 (TLR4) and plays a crucial role in the immune response to Gram-negative bacterial infections. It acts as an essential co-receptor for TLR4, forming a complex that recognizes lipopolysaccharide (LPS). MD2 facilitates the transfer of LPS to TLR4, leading to the activation of signaling pathways that result in the production of pro-inflammatory cytokines and chemokines. By enhancing the sensitivity of TLR4 to LPS, MD2 plays a key role in initiating and amplifying the innate immune response against bacterial pathogens, making it a vital component of the host's defense mechanisms.
CD14 is a glycoprotein primarily expressed on the surface of monocytes and macrophages, where it serves as a co-receptor for the recognition of pathogen-associated molecular patterns (PAMPs), particularly lipopolysaccharides (LPS) from Gram-negative bacteria. CD14 exists in two forms: a membrane-bound form and a soluble form. The membrane-bound CD14 facilitates the binding of LPS and other microbial components to the TLR4-MD2 complex, which is critical for initiating innate immune responses. This activation leads to the production of pro-inflammatory cytokines and helps mediate the body's response to infections and inflammation. CD14 also plays a role in modulating immune responses and maintaining immune homeostasis.