PharmaOnco™ CHO-Tg(Human BCMA//Gluc) Stable Cell Line, Single Clone

Creative Biolabs offers CHO-Tg(Human BCMA//Gluc) stable cell line which the BCMA gene stably expressed in CHO cells.

The CHO-Tg(Human BCMA//Gluc) stable cell line is a genetically engineered Chinese Hamster Ovary (CHO) cell line that has been transfected with a construct containing the gene for the human B-cell maturation antigen (BCMA) fused to the gene for Gaussia luciferase (Gluc). This stable cell line allows for the production of BCMA protein along with the secretion of Gluc, which can be used as a reporter gene for monitoring protein expression and secretion levels. This cell line is commonly used in research and drug development studies related to BCMA-targeted therapies for multiple myeloma and other B-cell malignancies.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

BCMA

Human

CHO

GenBank accession #NM_001192

The BCMA (B cell maturation antigen) gene is located on chromosome 16q23 and encodes a cell surface protein that is a member of the tumor necrosis factor receptor superfamily. BCMA is primarily expressed on the surface of plasma cells and activated memory B cells, and it plays a crucial role in the survival and function of these cells. BCMA interacts with its ligands BAFF (B cell activating factor) and APRIL (a proliferation-inducing ligand) to promote the survival and differentiation of plasma cells, as well as regulate the immune response. Dysregulation of BCMA signaling has been associated with various autoimmune diseases and cancers, making it a potential therapeutic target for these conditions. Mutations in the BCMA gene have been linked to multiple myeloma, a type of cancer that affects plasma cells, as well as other B cell-related disorders. Targeting BCMA with monoclonal antibodies and chimeric antigen receptor (CAR) T-cell therapies has shown promising results in the treatment of multiple myeloma and other B cell malignancies.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.