PharmaOnco™ HEK293-Tg(Human TDO) Stable Cell Line, Single Clone

Creative Biolabs offers HEK293-Tg(Human TDO) stable cell line which the TDO gene stably expressed in HEK293 cells.

The HEK293-Tg(Human TDO) stable cell line is a genetically modified cell line derived from the human embryonic kidney (HEK) 293 cell line. In this stable cell line, the gene for human tryptophan 2,3-dioxygenase (TDO), an enzyme involved in tryptophan metabolism, has been stably integrated into the genome. This cell line can be used for studying the role of TDO in tryptophan metabolism, as well as for screening potential inhibitors or activators of TDO activity. It may also have applications in drug discovery and development, particularly in the field of cancer research where TDO has been implicated in promoting tumor growth and immune evasion.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

TDO

Human

HEK293

Genbank accession number NM_005651.1

The TDO gene, also known as tryptophan 2,3-dioxygenase, is an enzyme that catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan metabolism. This pathway is important in regulating immune responses, inflammation, and neurotransmitter synthesis. TDO is primarily expressed in the liver, where it plays a key role in regulating tryptophan levels in the body. By converting tryptophan to kynurenine, TDO helps to regulate the balance of this essential amino acid in the body. Mutations in the TDO gene have been associated with various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases. Research into the TDO gene and its role in these conditions is ongoing, with the hope of developing new treatment strategies targeting this pathway.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.