PharmaOnco™ Jurkat-NFAT eGFP Reporter Cell Line, Single Clone

Creative Biolabs offers Jurkat-NFAT eGFP reporter cell line which has been engineered to express a reporter gene eGFP under the control of the NFAT promoter.

The Jurkat-NFAT eGFP reporter cell line is a specific type of cell line that has been engineered to express a reporter gene called enhanced green fluorescent protein (eGFP) under the control of the nuclear factor of activated T-cells (NFAT) promoter. NFAT is a transcription factor that plays a key role in regulating the activation of T-cells, which are important immune cells involved in the body's defense against infections and diseases. This cell line is often used in studies to investigate the molecular mechanisms underlying T-cell activation, signaling pathways, and immune responses.

Consistent gene expression;
Reliability and reproducibility;

Functional assay

Jurkat

NFAT eGFP reporter is a genetic construct that includes the Nuclear Factor of Activated T-cells (NFAT) promoter region linked to the enhanced green fluorescent protein (eGFP) gene. This reporter system is commonly used in cell biology research to monitor cellular activity of NFAT, a transcription factor that plays a critical role in regulating immune response and other biological processes. When activated, NFAT translocates to the nucleus and induces expression of eGFP, resulting in fluorescent signal that can be easily visualized and quantified using fluorescence microscopy or flow cytometry. The NFAT eGFP reporter system provides a convenient tool for studying NFAT activation dynamics in living cells and investigating the signaling pathways that control NFAT activity.

1 x 10e6 cells/vial, Frozen

Creative Biolabs provides sterility testing in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Identity testing is required for newly established cell lines. Isoenzyme analysis is used to confirm the identity of the species of a cell line. Alternative methods for identity testing include DNA fingerprinting, STR analysis and karyology.

A broad range of viruses is susceptible to affecting human cell lines. We can provide in vivo/vitro virus safety assays by utilizing various animal systems. These viruses include: adventitious viruses, bovine viruses, human and simian viruses, porcine viruses, retrovirus and rodent viruses.

We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Dry Ice

Frozen cells should be stored in a liquid nitrogen tank (-150°C~-190°C). After thawing, cells should be immediately transferred to the appropriate culture medium and maintained under recommended culture conditions.

Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Disclaimer: We cannot guarantee cell viability if the cells are not thawed immediately upon receipt and grown according to handling procedure.

Avoid multiple freeze/thaw cycles

For research use only, not for diagnostic or therapeutic use.

All of our stable cell lines meet the highest quality standards and have been confirmed to be pathogen-free.