Jurkat-Clone E6-1 In Vitro Reporter Gene (Green Fluorescence Protein) Assay

This assay is to provide Jurkat-Clone E6-1-based In Vitro Reporter Gene (Green Fluorescence Protein) Assay to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Jurkat-Clone E6-1

Human

Jurkat, Clone E6-1 is a clone of the Jurkat-FHCRC cell line, a derivative of the Jurkat cell line, which was established from the peripheral blood of a 14-year-old, male, acute T-cell leukemia patient. This cell line can be used in immune system disorder research and immunology and immuno-oncology research.

Acute Lymphoblastic Leukemia; ALL

Oncology

In Vitro Reporter Gene (Green Fluorescence Protein) Assay

Jurkat-Clone E6-1-cell based In Vitro Reporter Gene (Green Fluorescence Protein) Assay

Reporter gene assays have become a valuable technique for studying gene expression by both cis- and trans-acting regulatory factors. The action of a reporter gene is evaluated in a reporter gene assay and a reporter gene construct normally comprises of a target regulatory DNA sequence (usually a major in a cancer pathway) to be analyzed, sequence for the reported gene and a sequence for functional mRNA in an expression vector. The expression vector can be transfected into the desired cell type. After transfection of the vector, the effects on a drug on the expression of target gene can be evaluated. The ideal reported gene activity should detect with high sensitivity over any endogenous gene expression. Reporter gene systems permit the use of cancer pathway-specific or developmentally regulated regulatory DNA sequences as markers to analyze gene expression in specific pathways associated with molecular mechanisms of cancer.

Green Fluorescence Protein

High-throughput Screening, High Content Screening and Reporter Gene Assays