Mouse CXCL10 ELISA Kit, Lot 21AO-788 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Mouse CXCL10 in Cell Culture Supernatant, Plasma, Serum, Tissue Lysate.

For quantitative detection of CXCL10 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.

ELISA

Anti-CXCL10 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CXCL10 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of CXCL10 can be calculated.

CXCL10

Mouse

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Plasma, Serum, Tissue Lysate

1. One 96-well plate pre-coated with anti-mouse CXCL10 antibody 2. Lyophilized mouse CXCL10 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-mouse CXCL10 antibody (Concentrated): 130 µl.

1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

< 1 pg/mL

Pre-coated

1.Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination.

For Research Use only

Interferon Gamma Induced Protein 10kDa (IP10)

CXCL10; C7; IFI10; INP10; IP-10; SCYB10; crg-2; gIP-10; mob-1; CRG-2; IP10; Ifi10; Scyb10; C-X-C motif chemokine ligand 10; C-X-C motif chemokine 10; chemokine (C-X-C motif) ligand 10; CXCL10; cxl10; Cxcl10

C-X-C motif chemokine 10 (CXCL10) also known as Interferon gamma-induced protein 10 (IP-10) or small-inducible cytokine B10 is an 8.7 kDa protein that in humans is encoded by the CXCL10 gene.

15945

P17515