Specific quantification of acetoacetate (ketone body).
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Acetoacetate Assay Kit specifically quantifies acetoacetate, distinguishing it from other ketone bodies to provide precise monitoring of diabetic ketoacidosis. This assay supports clinical trials for ketogenic diets and metabolic therapies by tracking acute shifts in fatty acid oxidation. It is essential for evaluating the safety and efficacy of drugs that influence ketogenesis.
Features
Specific for acetoacetate (does not detect beta-hydroxybutyrate); Precise monitoring of ketoacidosis in clinical trials.
Process Relevance
Metabolic Monitoring (Ketosis)
Application Stage
Clinical Research
Applications
Monitoring ketosis in diabetes and ketogenic diet studies.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 550 nm
Sample Type
Serum, plasma, urine, and other biological samples
Research Areas
Diabetes & Obesity; Endocrinology
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 30 min
LOD
12 μM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Acetoacetate is the first ketone body produced during ketogenesis. Accurate specific measurement allows for the precise calculation of the ketone body ratio in metabolic states distinguishing it from beta-hydroxybutyrate.