OXPHOSight™ Isocitrate Assay Kit

CAT#: ITS-1125-HM111
Product Type: Assay Kit
Species: All Species
Short Description
Quantifies isocitrate concentrations.
Brand
OXPHOSight
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Isocitrate Assay Kit uses enzymatic determination to validate metabolic flow through the TCA cycle in bioenergetic studies. This assay quantifies isocitrate levels, helping to assess blockages or accelerations in mitochondrial respiration. It supports the investigation of IDH mutations and metabolic shifts in cancer, providing key data for metabolic flux analysis.
Features
Enzymatic determination; Validates metabolic flow through the TCA cycle in bioenergetic studies.
Process Relevance
TCA Cycle Flux Analysis
Application Stage
Basic Research
Applications
Metabolic flux analysis in the TCA cycle.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Food, beverage, serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Bioenergetics
Size
100 tests
Data Output Type
Quantitative (Concentration)
Assay Time
Results in 10 min
LOD
20 µM
Species
All Species
Storage
Store at −20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to −20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped on ice under temperature-controlled conditions.
Background
Isocitrate is a substrate for isocitrate dehydrogenase. Its accumulation or depletion reflects TCA cycle flux and is pertinent in studies of IDH mutations and mitochondrial bioenergetics.
For Research Use Only | Not For Clinical Use
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