Short Description
Quantifies isocitrate concentrations.
Development Stage
Research-Grade Metabolic Assay Development
Description
The OXPHOSight™ Isocitrate Assay Kit uses enzymatic determination to validate metabolic flow through the TCA cycle in bioenergetic studies. This assay quantifies isocitrate levels, helping to assess blockages or accelerations in mitochondrial respiration. It supports the investigation of IDH mutations and metabolic shifts in cancer, providing key data for metabolic flux analysis.
Features
Enzymatic determination; Validates metabolic flow through the TCA cycle in bioenergetic studies.
Process Relevance
TCA Cycle Flux Analysis
Application Stage
Basic Research
Applications
Metabolic flux analysis in the TCA cycle.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 565 nm
Sample Type
Food, beverage, serum, citrate-plasma, urine, cell lysate, culture medium
Research Areas
Metabolic Disease; Bioenergetics
Datasheet