Pig IL1RN ELISA Kit, Lot 21AO-1294 [Cancer Immune Checkpoint Assay Kit]

CAT#: IOK-05-P1205
Product Type: ELISA Kit
Target: IL1RN
Short Description
The kit is designed for in vitro quantitative measurement of Pig IL1RN in Plasma, Serum, Tissue Homogenate.
Description
For the quantitative determination of pig interleukin 1 receptor antagonist (IL-1ra) concentrations in serum, plasma, tissue homogenates.
Applications
ELISA
Application Notes
Optimal working dilution should be determined by the investigator.
Target
IL1RN
Reactivity
Pig
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Analytical Method
Quantitative
Sample Type
Plasma, Serum, Tissue Homogenate
Specificity
This assay has high sensitivity and excellent specificity for detection of pig IL-1ra. No significant cross-reactivity or interference between pig IL-1ra and analogues was observed.
Cross-Reactivity
Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pig IL-1ra and all the analogues, therefore, cross reaction may still exist.
Components
Assay plate
Standard
HRP-avidin (100 x concentrate)
Biotin-antibody (100 x concentrate)
Sample Diluent
HRP-avidin Diluent
Biotin-antibody Diluent
Wash Buffer (25 x concentrate)
TMB Substrate
Stop Solution
Adhesive Strip
Sensitivity
15.6 pg/mL
Sample Volume
100 μL
Assay Time
1 - 4.5 h
Plate
Pre-coated
Reagent Preparation
Biotin-antibody (1×) - Centrifuge the vial before opening.
HRP-avidin (1×) - Centrifuge the vial before opening.
Wash Buffer (1×) - If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved.
Standard - Centrifuge the standard vial at 6000-10000rpm for 30s.
Assay Precision
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess.
Storage
4 °C/-20 °C
Storage Comment
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8°C.
Expiry Date
6 months
Note
Unopened kit Store at 2 - 8°C. Do not use the kit beyond the expiration date. May be stored for up to 1 month at 2 - 8°C. Coated assay Try to keep it in a sealed aluminum foil bag, plate and avoid the damp. Standard May be stored for up to 1 month at 2 - 8° C.
Restrictions
For Research Use only
Alternative Name
Interleukin 1 Receptor Antagonist (IL1RA)
Synonyms
DIRA; ICIL-1RA; IL-1RN; IL-1ra; IL-1ra3; IL1F3; IL1RA; IRAP; MVCD4; IRAP1; IL1RN; IL-1RA; F630041P17Rik; interleukin 1 receptor antagonist; interleukin 1 receptor antagonist-like; IL1RN; Il1rn; IL1RNL
Background
Abbreviation: IL1RN
Alias: DIRA, ICIL-1RA, IL-1ra3, IL1F3, IL1RA, IRAP, MGC10430, MVCD4, IL1RN (IL1F3)|OTTHUMP00000203730|intracellular IL-1 receptor antagonist type II|intracellular interleukin-1 receptor antagonist (icIL-1r)
Gene ID
397499
UniProt
Q29056
Pathways
NF-kappaB Signaling, Hormone Transport, Cancer Immune Checkpoints
For Research Use Only | Not For Clinical Use
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