Short Description
Quantifies target protein phosphorylation levels using an ELISA-based colorimetric method.
Development Stage
Research-Grade Signal Pathway Assay Development
Description
The SigPath™ Human/Mouse RB(T821/826) Phosphorylation ELISA Kit provides a rapid and sensitive solution for quantifying phosphorylated pRB(T821/826) directly in cultured cells. This cell-based system eliminates the need for lysate preparation, utilizing in-cell normalization to total protein to ensure high precision. It is an essential tool for high-throughput screening of cell cycle modulators and drug candidates in cancer and signal transduction research.
Features
Elite Sensitivity & Range: Employs a high-affinity sandwich format to detect endogenous phosphorylated RB with a wide dynamic range and low-nanogram sensitivity.
Dual-Species Versatility: Validated for both human and mouse cell lysates, ensuring seamless cross-model consistency for translational oncology and fundamental research.
Simplified Plate-Based Workflow: Streamlines the quantification of site-specific phosphorylation (T821/826) in a standard 96-well format, significantly reducing processing time.
Rigorous Specificity: Utilizes proprietary antibody pairs that exclusively recognize the T821/826 phospho-epitope without cross-reactivity to total or non-phosphorylated Retinoblastoma protein.
Process Relevance
Monitoring cell cycle progression to support the screening of CDK inhibitors and validate anti-proliferative therapeutic efficacy.
Application Stage
Lead discovery, mechanism of action studies, and preclinical evaluation of cell cycle-targeted drug candidates.
Applications
Quantifying site-specific phosphorylation in cellular assays, screening chemical libraries for kinase inhibition, and analyzing growth factor signaling pathways in human and murine cell lines.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Target
Phosphorylated retinoblastoma protein at threonine 821 and threonine 826 residues.
Detection Method
Fluorescence Detection (Ex 530 nm / Em 585 nm or Ex 360 nm / Em 457 nm)
Research Areas
Oncology therapeutics, cell cycle regulation, developmental biology, senescence studies, and regenerative medicine, etc.
Datasheet