Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide SK-ES-1-based In Vitro ATP Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
SK-ES-1
Target Cell Organism
Human
Target Cell Background
SK-ES-1 is a human Ewing sarcoma (anaplastic osteosarcoma) cell line that displays epithelial morphology and grows in adherent tissue culture.
Related Diseases
Ewing Sarcoma
Research Area
Oncology
Assay Name
In Vitro ATP Assay (Cell Viability)
Short Description
SK-ES-1-cell based In Vitro ATP Assay (Cell Viability)
Assay Description
Adenosine triphosphate (ATP) assay is another widely used cell viability assay. When the cancer cells undergo apoptosis or necrosis upon treatment with a compound, cellular ATP content decreases and it can be quantified by colorimetric, fluorometric or luminometric way. The latest bioluminescence assays measure cellular ATP content using the enzyme luciferase which is more sensitive and reproducible when determining cell viability compared to conventional assays.
Assay Alternative Names
Adenosine Triphosphate Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins