A549 In Vitro Reactive Nitrogen Species Assay (Oxidative Stress)

This assay is to provide A549-based In Vitro Reactive Nitrogen Species Assay (Oxidative Stress) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

A549

Human

A549 cells were isolated from the lung tissue of a White, 58-year-old male with lung cancer. This cell line can be used in cancer, immuno-oncology and toxicology research.

Lung Carcinoma

Oncology

In Vitro Reactive Nitrogen Species Assay (Oxidative Stress)

A549-cell based In Vitro Reactive Nitrogen Species Assay (Oxidative Stress)

The production of reactive nitrogen species can also occur as a result of oxidative stress. Levels of nitrite (NO2-) and nitrate (NO3-) can be measured in cell lysates, plasma and urine as an oxidative stress marker and several nitrate/nitrite assay kits have been developed to measure nitrite (NO2-) and nitrate (NO3-) levels.

Oxidative Stress Assays

Disturbance between the production of reactive oxygen species (ROS), free radicals and antioxidant mechanisms is defined as the oxidative stress, or more precisely, it is an imbalance between the oxidant and antioxidant state in cells. This imbalance can cause harmful effects to cells and biomolecules, which ultimately causes adverse effects in the whole organism. Oxidative imbalance can target important proteinsand lipids in cells, which can increase the risk of developing a cancer. On the other hand, increased ROS production in cancer cells by certain cancer drugs can also arrest cancer cell cycle and cause senescence and apoptosis through oxidative stress.

Markers Thiobarbituric Acid Reactive Substances