Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide A549-based In Vitro SRB Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
A549
Target Cell Organism
Human
Target Cell Background
A549 cells were isolated from the lung tissue of a White, 58-year-old male with lung cancer. This cell line can be used in cancer, immuno-oncology and toxicology research.
Related Diseases
Lung Carcinoma
Research Area
Oncology
Assay Name
In Vitro SRB Assay (Cell Viability)
Short Description
A549-cell based In Vitro SRB Assay (Cell Viability)
Assay Description
The sulforhodamine B is another common colorimetric assay that is used to investigate viability of cancer cells. The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500-540 nm.
Assay Alternative Names
Sulforhodamine B Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins