B16F10 In Vitro SRB Assay (Cell Viability)

CAT#: ITS-1022-YF8
Target Cell Organism: Mouse
Target Cell Alternative Name: B16-F10
Target Cell Name: B16F10
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide B16F10-based In Vitro SRB Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
B16F10
Target Cell Organism
Mouse
Target Cell Background
B16F10 cell line (from Mus musculus C57BL/6J strain) is a convenient and widely used experimental model of highly metastatic melanoma to study cytotoxicity, migration, metastatic spread and tissue invasion. B16-F10 is a cell line exhibiting a morphology of spindle-shaped and epithelial-like cells that was isolated from skin tissue of a mouse with melanoma. Use these cells in your skin cancer research.
Target Cell Alternative Name
B16-F10
Related Diseases
Melanoma
Research Area
Oncology
Assay Name
In Vitro SRB Assay (Cell Viability)
Short Description
B16F10-cell based In Vitro SRB Assay (Cell Viability)
Assay Description
The sulforhodamine B is another common colorimetric assay that is used to investigate viability of cancer cells. The dye SRB can bind with proteins in viable cells under mild acidic conditions, and the bound dye is finally solubilized to get absorbance at 500-540 nm.
Assay Alternative Names
Sulforhodamine B Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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