C2BBe1 In Vitro PI Assay (Cell Viability)

CAT#: ITS-1022-YF5372
Target Cell Organism: Human
Target Cell Name: C2BBe1
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide C2BBe1-based In Vitro PI Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
C2BBe1
Target Cell Organism
Human
Target Cell Background
The C2BBe1 (brush border expressing) cell line was cloned in 1988 from the Caco-2 cell line by limiting dilution.
The clone was selected on the basis of morphological homogeneity and exclusive apical villin localization.
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro PI Assay (Cell Viability)
Short Description
C2BBe1-cell based In Vitro PI Assay (Cell Viability)
Assay Description
Propidium iodide (PI) is another dye that can be used to distinguish viable and non-viable cells. PI can only bind with DNA of cells with damaged cell membranes and it cannot enter viable cells. Uptake of PI into cells can be monitored by flow cytometry (FCM) or fluorescence microscopy.
Assay Alternative Names
Propidium Iodide Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Dye Exclusion Assays
For Research Use Only | Not For Clinical Use
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