Caco-2 In Vitro PI Assay (Cell Viability)

CAT#: ITS-1022-YF5477
Target Cell Organism: Human
Target Cell Alternative Name: Caco2
Target Cell Name: Caco-2
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide Caco-2-based In Vitro PI Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
Caco-2
Target Cell Organism
Human
Target Cell Background
Caco-2 [Caco2] are epithelial cells isolated from colon tissue derived from a 72-year-old, White, male with colorectal adenocarcinoma. This cell line is a suitable transfection host and has applications in cancer and toxicology research.
Target Cell Alternative Name
Caco2
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro PI Assay (Cell Viability)
Short Description
Caco-2-cell based In Vitro PI Assay (Cell Viability)
Assay Description
Propidium iodide (PI) is another dye that can be used to distinguish viable and non-viable cells. PI can only bind with DNA of cells with damaged cell membranes and it cannot enter viable cells. Uptake of PI into cells can be monitored by flow cytometry (FCM) or fluorescence microscopy.
Assay Alternative Names
Propidium Iodide Assay
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Dye Exclusion Assays
For Research Use Only | Not For Clinical Use
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