CAL 27 In Vitro RNA Expression Analysis Assay (Cap Analysis)
CAT#: ITS-1122-YF3345
Target Cell Organism: Human
Target Cell Name: CAL 27
Assay Type: Gene and Protein Expression Assays
Assay Overview
This assay is to provide CAL 27-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
CAL 27
Target Cell Organism
Human
Target Cell Background
Cal 27 cells are epithelial cells isolated from tissue taken prior to treatment from a 56-year-old, White male with a lesion in the middle of the tongue in 1982.
Related Diseases
Squamous Cell Carcinoma
Research Area
Oncology
Assay Name
In Vitro RNA Expression Analysis Assay (Cap Analysis)
Short Description
CAL 27-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)
Assay Description
Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression tech nique has become a useful platform.
Assay Type
Gene and Protein Expression Assays
Assay Type Details
Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.