As key players in cellular function, calcium channels are critical targets for drug discovery, especially for therapeutic antibodies and proteins. Creative Biolabs offers a comprehensive calcium ion channel assay service to facilitate the development of therapeutic antibodies and proteins targeting these essential channels.

Understanding the Role of Calcium Ion Channels in Drug Development

Calcium ion channels are key regulators of intracellular calcium concentration, a vital second messenger that controls various cellular functions. These channels are involved in numerous biological processes, including muscle contraction, neuronal transmission, hormone secretion, and gene expression. Calcium ion channels are divided into several subtypes, such as L-type, N-type, P/Q-type, and T-type, each having distinct distribution patterns and functions within the body. Given their critical functions, calcium ion channels are prominent targets in drug discovery, especially for the development of therapies addressing neurological disorders, cardiovascular diseases, and immune system dysfunctions.

Fig.1 Types of calcium channels.¹

Advanced Calcium Ion Channel Assay Service

At Creative Biolabs, our calcium ion channel assay service is designed to provide accurate, reliable, and reproducible data for drug discovery and development. Our electrophysiology team has developed a variety of calcium ion channel cell lines, tailored to enable precise screening of therapeutic candidates. We provide customized assays for a broad range of ion channel targets, including CaV1.2, Cav1.3, CaV2.2, CaV2.1, CaV3.2, CRAC (STIM1/Orai). By utilizing advanced patch-clamp techniques, we offer highly sensitive and real-time analysis of calcium ion channel activity. This allows us to measure both the gating properties and functional responses of calcium ion channels to various compounds, including antibodies, proteins, and small molecules. Whether you are screening for potential drug candidates, assessing their potency, or studying their interaction with specific ion channel subtypes, our Calcium Ion Channel Assay provides the insights you need to advance your research.

Technological Excellence for In-Depth Analysis

Our assay service is built on a foundation of advanced technologies, including:

• Manual and Automated Patch Clamp Techniques: Recognized as the gold standard in ion channel screening, these techniques allow us to measure ionic currents with high precision. While manual patch clamp assays deliver unparalleled detail, automated systems provide the throughput necessary for large-scale screenings.
• Fluorometric Imaging Plate Reader Technology: This high-throughput system enables real-time monitoring of calcium flux and membrane potential changes. By providing rapid and sensitive assessments, this technology facilitates the swift identification of potential therapeutic candidates.
• High-Throughput Screening: Our Calcium Ion Channel Assay Service is optimized for high-throughput screening, enabling rapid evaluation of large compound libraries. This makes it an ideal solution for identifying potential therapeutic candidates early in the development process.

Advantages

• Broad Target Coverage
  We provide assays for key calcium ion channel subtypes, including CaV1.2, CaV1.3, CaV2.1, CaV2.2, CaV3.2, and CRAC. This supports research across various therapeutic areas such as neurology, cardiology, and immunology.
• Advanced Assay Technologies
  Our service integrates manual and automated patch clamp systems with fluorometric imaging plate readers, enabling both high-precision electrophysiological data and efficient high-throughput compound screening.
• Customizable Assay Design
  Assays are tailored to each client's specific needs, including cell line selection and assay condition optimization, ensuring reliable results aligned with drug development goals.
• Real-Time Functional Insights
  We provide real-time analysis of calcium ion channel activity, offering detailed data on gating behavior and compound effects, essential for drug potency and mechanism-of-action studies.

Whether you're looking to identify novel therapeutic targets, optimize drug candidates, or conduct functional validation, Creative Biolabs' calcium ion channel assay service provides the robust platform you need to move your research forward with confidence. For more information about our services or to discuss your specific project needs, feel free to contact us today.

Reference

1. Neely, Alan, and Patricia Hidalgo. "Structure-function of proteins interacting with the α1 pore-forming subunit of high-voltage-activated calcium channels." Frontiers in physiology 5 (2014): 209. Distributed under Open Access license CC BY 3.0, without modification.

For Research Use Only | Not For Clinical Use