CertiQ™ Ammonia Assay Kit

CAT#: ITS-1125-YL45
Product Type: Assay Kit
Species: All Species
Brand: CertiQ
Development Stage: Research-Grade Quality Control Assay Development
Short Description
Quantifies ammonia using a colorimetric method.
Brand
CertiQ
Development Stage
Research-Grade Quality Control Assay Development
Description
This CertiQ™ Ammonia Assay Kit provides a sensitive colorimetric method for measuring ammonia levels in biological fluids and experimental samples. Utilizing a modified Berthelot reaction, the system produces a stable colored product proportional to the ammonia concentration. This reliable tool facilitates research into nitrogen metabolism and amino acid degradation, offering high sensitivity and minimal interference for fundamental studies in metabolic regulation and physiological biochemical profiling.
Features
Employs a specific glutamate dehydrogenase reaction for the accurate quantification of ammonia, facilitating high-sensitivity research into nitrogen metabolism and urea cycle function in biological fluids.
Process Relevance
Quantifying ammonia levels to track nitrogen metabolic flux and optimize nutrient supply in cultures.
Application Stage
Nitrogen metabolism research and bioprocess monitoring.
Applications
Quantify ammonia concentrations in biological fluids and cell media to analyze nitrogen metabolism and cellular waste processing in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 360 nm / Em 450 nm)
Sample Type
Serum, plasma, environmental water, soil extracts.
Research Areas
Nitrogen Metabolism; Biochemistry; Microbiology
Size
200 tests
Data Output Type
Quantitative concentration measurement
Assay Time
15 min
LOD
LOD: 12 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Ammonia is a byproduct of amino acid metabolism and is highly toxic at elevated levels. Its efficient conversion and excretion are essential for maintaining physiological pH and preventing neurotoxicity.
For Research Use Only | Not For Clinical Use
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