Short Description
Quantifies ammonia using a colorimetric method.
Development Stage
Research-Grade Quality Control Assay Development
Description
This CertiQ™ Ammonia Assay Kit provides a sensitive colorimetric method for measuring ammonia levels in biological fluids and experimental samples. Utilizing a modified Berthelot reaction, the system produces a stable colored product proportional to the ammonia concentration. This reliable tool facilitates research into nitrogen metabolism and amino acid degradation, offering high sensitivity and minimal interference for fundamental studies in metabolic regulation and physiological biochemical profiling.
Features
Employs a specific glutamate dehydrogenase reaction for the accurate quantification of ammonia, facilitating high-sensitivity research into nitrogen metabolism and urea cycle function in biological fluids.
Process Relevance
Quantifying ammonia levels to track nitrogen metabolic flux and optimize nutrient supply in cultures.
Application Stage
Nitrogen metabolism research and bioprocess monitoring.
Applications
Quantify ammonia concentrations in biological fluids and cell media to analyze nitrogen metabolism and cellular waste processing in research.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Fluorescence Detection (Ex 360 nm / Em 450 nm)
Sample Type
Serum, plasma, environmental water, soil extracts.
Research Areas
Nitrogen Metabolism; Biochemistry; Microbiology
Datasheet