CertiQ™ Detergent Assay Kit

CAT#: ITS-1125-YL43
Product Type: Assay Kit
Species: All Species
Brand: CertiQ
Development Stage: Research-Grade Quality Control Assay Development
Short Description
Quantifies detergent concentration using a colorimetric method.
Brand
CertiQ
Development Stage
Research-Grade Quality Control Assay Development
Description
This CertiQ™ Detergent Assay Kit provides a streamlined colorimetric method for quantifying common laboratory detergents in biological preparations and buffer systems. The system utilizes a specific dye-binding technique that produces a measurable spectral shift proportional to the detergent concentration. This analytical tool is essential for researchers monitoring protein purification efficiency and sample quality, ensuring high sensitivity and reliable quantification for fundamental biochemical and molecular research.
Features
Employs a specific dye-binding method for the rapid quantification of common detergents, facilitating effective monitoring of detergent removal and quality control during complex protein purification workflows.
Process Relevance
Quantifying residual detergents to verify purification efficiency and prevent interference in downstream biological assays.
Application Stage
Protein purification and detergent removal monitoring.
Applications
Measure residual detergent concentrations in purified protein samples to ensure compatibility with downstream biochemical and structural analysis.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 560nm and 650nm
Sample Type
Protein solutions, biological buffers, environmental water.
Research Areas
Protein Purification; Quality Control; Proteomics
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
15 min
LOD
LOD: Tween 80: 0.012 to 4 mM; Tween 20: 0.06 to 5 mM; Triton X-100: 0.23 to 12 mM; Brij L23/35: 0.09 to 5 mM; DTAC: 0.08 to 2 mM; SDS: 10 to 25 mM
Species
All Species
Storage
Store at 4 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. Do not store at elevated temperatures for extended periods.
Expiry Date
12 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
Detergents are widely used in biochemical research for membrane protein extraction and sample solubilization. However, their presence can interfere with various downstream applications, making their quantification and removal critical for successful experimentation.
For Research Use Only | Not For Clinical Use
Online Inquiry