COLO 205 In Vitro MTS Assay (Cell Viability)

This assay is to provide COLO 205-based In Vitro MTS Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

COLO 205

Human

The COLO 205 cell line is made up of epithelial cells isolated in 1975 from ascitic fluid derived from a 70-year-old, White, male with colon cancer. The cells can be used for cancer and toxicology research.

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro MTS Assay (Cell Viability)

COLO 205-cell based In Vitro MTS Assay (Cell Viability)

Apart from the MTT tetrazolium, other tetrazolium compounds such as MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) can also generate formazan upon reduction by oxidoreductase or dehydrogenase. Unlike the normal MTT assay, assays with these regents (MTS, XTT and WST) do not require formazan solubilization step as solubilization occurs in the cell medium itself. Therefore, XTT, WST and MTS reagents can be used to assay viability of cells, which do not require a surface to attach.

MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) Assay

Cell Viability/Cytotoxicity and Antiproliferative Assays

Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.

Cell Viability Assay

Cell-based Viability Assays Based on Cellular Enzymes and Proteins