Colo205 In Vitro RNA Expression Analysis Assay (DNA Microarray)

This assay is to provide Colo205-based In Vitro RNA Expression Analysis Assay (DNA Microarray) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

Colo205

Human

The COLO 205 cell line is made up of epithelial cells isolated in 1975 from ascitic fluid derived from a White, 70-year-old, man with colon cancer. The cells can be used for cancer and toxicology research.

COLO 205

Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (DNA Microarray)

Colo205-cell based In Vitro RNA Expression Analysis Assay (DNA Microarray)

DNA microarray is another technique that enables the analysis of gene expression. Fluorescent labeled cDNA (with cyanines) synthesized from a test sample is hybridized to target probes with complementary sequences attached on microarray chips and strongly bound complexes are screened after scanning optically.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.