Description
Three-reagent kit for staining amyloids, characteristic by its high ionic strength and pH enhancing the specificity of Congo Red dye binding to amyloid clusters.
Congo Red Puchtler kit is used for staining amyloids (amorphous clusters) in histology sections. Despite their variable protein composition, amyloid deposits are linked by fibril beta-sheets formation. Congo Red dye binds to fibrils and form a regular pattern of dye molecules during the process. The structural regularity of the dye under polarized light shows double refraction of light by displaying green coloration. High pH level and ionic strength of solutions according to Puchtler achieve higher dye specificity to amyloid
Result
Amyloid deposits - brick red, green under polarized light
Collagen - bright red, grey-silver under polarized light
Nuclei - blue
Resources
Special stains Kit - All Products
Applications
Tissue Stainning
Datasheet