CT26.WT In Vitro RNA Expression Analysis Assay (Cap Analysis)
CAT#: ITS-1122-YF1140
Target Cell Organism: Mouse
Target Cell Alternative Name: CT26
Target Cell Name: CT26.WT
Assay Type: Gene and Protein Expression Assays
Assay Overview
This assay is to provide CT26.WT-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
CT26.WT
Target Cell Organism
Mouse
Target Cell Background
CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT. Use CT26.WT mouse fibroblasts with CT26.CL25 as a model for testing immunotherapy protocols and in studies on the host immune response.
Target Cell Alternative Name
CT26
Related Diseases
Colorectal Adenocarcinoma; Colon Cancer
Research Area
Oncology
Assay Name
In Vitro RNA Expression Analysis Assay (Cap Analysis)
Short Description
CT26.WT-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)
Assay Description
Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression tech nique has become a useful platform.
Assay Type
Gene and Protein Expression Assays
Assay Type Details
Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.