CT26.WT In Vitro RNA Expression Analysis Assay (SAGE)

This assay is to provide CT26.WT-based In Vitro RNA Expression Analysis Assay (SAGE) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

CT26.WT

Mouse

CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT. Use CT26.WT mouse fibroblasts with CT26.CL25 as a model for testing immunotherapy protocols and in studies on the host immune response.

CT26

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro RNA Expression Analysis Assay (SAGE)

CT26.WT-cell based In Vitro RNA Expression Analysis Assay (SAGE)

Serial analysis of gene expression (SAGE) is another molecular biological technique used to analyze gene expression. In this technique, mRNA extracted from the sample is reverse transcribed into cDNA and the resulting cDNA is then digested by restriction enzymes to produce tags. These tags are amplified by PCR and subjected to sequencing. Several modifications to SAGE such as Long SAGE, RL-SAGE and Super SAGE have been introduced to improve the SAGE technique.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.