CT26.WT In Vitro Scratch Assay (Migration)

This assay is to provide CT26.WT-based In Vitro Scratch Assay (Migration) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

CT26.WT

Mouse

CT26 is an N-nitroso-N-methylurethane-(NNMU) induced, undifferentiated colon carcinoma cell line. It was cloned to generate the cell line designated CT26.WT. Use CT26.WT mouse fibroblasts with CT26.CL25 as a model for testing immunotherapy protocols and in studies on the host immune response.

CT26

Colorectal Adenocarcinoma; Colon Cancer

Oncology

In Vitro Scratch Assay (Migration)

CT26.WT-cell based In Vitro Scratch Assay (Migration)

A wound healing assay is one of the simple and common assays used to monitor cancer cell migration. In a normal wound healing assay, attached cancer cells are exposed to tested compound (s) and incubated for 1-2 hours. A scratch is then manually made using a sterile pipette tip or needle and the width of the scratch is recorded at different time intervals. The effects of a testing drug on invasiveness of cells can also be monitored using this assay. However, this assay cannot be carried out with non-adherent cells.

Wound Healing Assay

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two dif ferent processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay