Custom Human CD2 Sandwich ELISA Kit-qPCR

CAT#: TM-HMM178
Product Type: Sandwich ELISA kit
Target: CD2
Reactivity: Human
Applications: CD2 detection
Description
This product is an innovative new assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR.
Features
High sensitivity qPCR readout for the quantitative measurement of human CD2 in serum, plasma, and cell culture supernatants.
Applications
CD2 detection
Target
CD2
Reactivity
Human
Detection Method
qPCR
Method Type
Quantitative
Sample Type
Cell culture supernatants, plasma, serum
Size
96-well strip plate
Receptor Family
Immunoglobulin superfamily
Qualified With
Certificate of quality
Plate
1, 2, or 5
Reagent Preparation
Real-time PCR instrument, Bio-Rad recommended; Precision pipettes to deliver 2 µL to 1 mL volumes; Adjustable 1-25 mL pipettes for reagent preparation; 100 mL and 1 liter graduated cylinders; Absorbent paper; Distilled or deionized water; Log-log graph paper or computer and software for data analysis; Tubes to prepare standard or sample dilutions; Heating block or water bath capable of 80°C
Assay Procedure
Prepare all reagents, samples and standards as instructed; Add 25µL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C; Add 25 µL detection affinity reagent to each well; Incubate 1 hour at room temperature; Add 25µL of Detection Reagent to each well; Incubate 1 hour; Add 15µL Primer solution 10µL of PCR master mix to each well; Run real-time PCR
Target/Description
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
Accession Number
P06729
Storage
Stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution.
Restrictions
For research use only. Intended for use by laboratory professionals.
Alternative Name
CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2
Synonyms
CD2; CD2 antigen; Erythrocyte receptor; LFA-3 receptor; LFA-2; Lymphocyte-function antigen-2; T11; SRBC; CD2 molecule; Rosette receptor; T-cell surface antigen CD2
Background
CD2 (cluster of differentiation 2) is a cell adhesion molecule found on the surface of T cells and natural killer (NK) cells. It has also been called T-cell surface antigen T11/Leu-5, LFA-2, LFA-3 receptor, erythrocyte receptor and rosette receptor.
For Research Use Only | Not For Clinical Use
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