Dog PD-L1 (CD274) ELISA Kit, Lot 21AO-105 [Cancer Immune Checkpoint Assay Kit]

The kit is designed for in vitro quantitative measurement of Dog PD-L1 (CD274) in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.

Canine PD-L1/CD274 ELISA Kit.

ELISA

Optimal working dilution should be determined by the investigator.

PD-L1 (CD274)

Dog

Colorimetric

Sandwich ELISA

Quantitative

Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 μL volumes
Adjustable 1-25 μL pipettes for reagent preparation
100 μL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Pre-coated

6 months

For Research Use only

Programmed Cell Death Protein 1 Ligand 1 (PDL1)

B7-H; B7H1; PD-L1; PDCD1L1; PDCD1LG1; PDL1; A530045L16Rik; B7h1; Pdcd1l1; Pdcd1lg1; Pdl1; RGD1566211; CD274 molecule; CD274 antigen; programmed cell death 1 ligand 2; CD274; Cd274; PDCD1LG2

484186

E2RKZ5

Cancer Immune Checkpoints

1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μL of standard or sample to each well.
3.Incubate 2.5 h at RT or O/N at 4°C.
4.Add 100 μL of prepared biotin antibody to each well.
5.Incubate 1 h at RT.
6.Add 100 μL of prepared Streptavidin solution to each well.
7.Incubate 45 min at RT.
8.Add 100 μL of TMB One-Step Substrate Reagent to each well.
9.Incubate 30 min at RT.
10.Add 50 μL of Stop Solution to each well.
11.Read at 450 nm immediately.