Deubiquitinating Enzyme (DUB) Assay Service
Creative Biolabs provides a comprehensive DUB Assay Service to enable detailed analysis of DUB activity, inhibitor screening, and functional assessment in immune-oncology research. Our customizable platforms support biochemical and cell-based evaluation of DUB function across diverse immunological pathways.
Background
Deubiquitinating enzymes (DUBs) are critical components of the ubiquitin–proteasome system, responsible for removing ubiquitin moieties from target proteins. In cancer biology, DUBs regulate protein stability, immune checkpoint expression, antigen presentation, and NF-κB signaling. Specific DUBs such as USP7, CYLD, A20, and OTUB1 have been implicated in the suppression of immune responses, T cell exhaustion, and immune tolerance mechanisms within tumors.
Featured Services at Creative Biolabs
Our DUB assays enable researchers to study the role of deubiquitination in immune regulation and tumor progression:
Fluorogenic DUB Activity Assay
Use of ubiquitin-AMC or Ub-rhodamine substrates to measure enzymatic cleavage activity with high sensitivity and real-time kinetics
DUB Isoform Profiling
Selective detection of specific DUB families (e.g., USP, UCH, OTU, MJD) using tailored substrates and inhibitors
Ubiquitin Chain Editing Assays
Analysis of DUB specificity toward K48-, K63-, and linear polyubiquitin chains relevant to immune pathway modulation
Cell-Based DUB Function Assays
Measurement of DUB impact on stability of immune modulators (e.g., PD-L1, IRF3, NF-κB, cGAS-STING components)
Target Validation
Functional depletion of DUBs combined with activity monitoring in immune-tumor co-cultures
High-Throughput Screening
Adaptable for 96/384-well formats using automated liquid handling and robust controls
Workflow
Highlights
- Immune-Focused Applications – Characterization of DUBs regulating immune checkpoint degradation and inflammatory responses
- Polyubiquitin Chain Discrimination – Chain-type specificity for understanding immune signaling outcomes (e.g., NF-κB vs. proteasome)
- Isoform-Specific Sensitivity – Enables selective profiling of catalytic activity across DUB families relevant to cancer immunology
- Functional Readouts – Measures impact on tumor antigen expression, cytokine regulation, and immunosuppressive pathways
- Screening & Validation Ready – Platform supports inhibitor identification, mechanistic elucidation, and lead validation
FAQs
Q1: Which DUBs can you analyze?
A1: We support major DUB families including USPs, UCHs, OTUs, JAMMs, and MJDs, with both generic and specific substrates.
Q2: Do you provide K48- and K63-linked polyubiquitin chain assays?
A2: Yes. We can test substrate preferences and chain-cleavage activities using biochemically defined ubiquitin chains.
Q3: Can DUB assays be integrated with immune cell functional studies?
A3: Certainly. We support co-culture assays to monitor DUB effects on immune checkpoint turnover and inflammatory gene expression.
Q4: Are your assays compatible with inhibitor screening?
A4: Yes. Our platform offers high-throughput DUB inhibitor screening with kinetic readouts, IC₅₀ determination, and Z′-factor validation.
Q5: How do you validate findings in immune-relevant models?
A5: We offer knockdown systems and reporter assays in primary or transformed immune cells to confirm DUB function.
Explore Deubiquitination Pathways in Immune Regulation with Creative Biolabs
We support researchers in decoding the ubiquitin landscape that shapes immune–tumor interactions. Our assay service enables actionable insights into immune checkpoint regulation, DUB-targeted therapy discovery, and immune escape mechanisms.
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