Description
Four-reagent kit for rapid staining of collagen fibers
Elastica-Van Gieson kit is used for staining elastin, connective tissue and collagen. Elastic fibers consist of elastin polymers and elastic microfibrilsthat make up a 3D network in an extracellular matrix inside connective tissue (skin, elastic cartilage, vascular walls, lung tissue and in vocal cords).
Unlike standard histology stains, Weigert Van Gieson reagent (known as resorcin-fuchsin reagent) displays selective differentiation of tissue samples,even in early phase of disease. When using Elastica-Van Gieson kit, the sections are first treated with Resorcine Fuchsin reagent. Positively chargedhydrophobic resorcin-fuchsin dye is present in large amounts and it is deposited owing to electropolarity to acid, negatively charged shells of elasticfibers.
After differentiation in diluted alcohol or rinsing in tap water, the nuclei are stained with acid-fast Weigert hematoxylin. The last phase of staining is staining with Fuchsin Acid Van Gieson reagent that contains two dyes (acid fuchsin, picric acid) that simultaneously stain different tissue structures. Acid fuchsin stains collagen fibers intensive red while picric acid stains muscle fibers, erythrocytes and glial fibers yellow. Sections are optimally stained in short period of time by using rapid staining method
Result
Black-blue - nuclei
Hues of red-pink - collagen
Dark purple-black - elastic fibers
Yellow - connective tissue, erythrocytes and muscle tissues
Resources
Special stains Kit - All Products
Applications
Tissue Stainning
Datasheet