ErbB Receptor Quantification Service
Our ErbB receptor quantification service provides precise measurement and analysis of ErbB family receptors' expression levels in biological samples, aiding in research and diagnostic applications related to cancer and other diseases.
ErbB Receptor
ErbB receptors, also known as epidermal growth factor receptor (EGFR) family receptors, are a group of closely related proteins that play critical roles in cell growth, differentiation, and survival. They are receptor tyrosine kinases that are activated by binding to specific ligands, such as growth factors. The ErbB family consists of four main members:
EGFR (ErbB1): This receptor is widely studied and is involved in the control of cell proliferation, migration, and survival. Aberrant activation of EGFR is associated with various cancers (e.g., lung, breast).
ErbB2 (HER2/neu): This receptor does not have a known ligand and is often overexpressed in certain cancers, particularly breast cancer. It forms heterodimers with other ErbB family members, enhancing their signaling capabilities.
ErbB3 (HER3): This receptor has reduced kinase activity and relies on heterodimerization with other ErbB receptors for effective signaling. It has been implicated in tumor growth and survival.
ErbB4 (HER4): This receptor can have both growth-promoting and growth-inhibiting effects, depending on the context. It can also be involved in development and differentiation processes.
The activation of ErbB receptors typically leads to a cascade of downstream signaling pathways, including the RAS/RAF/MEK/ERK pathway and the PI3K/AKT pathway, which are crucial for promoting cell survival, growth, and differentiation.
ErbB Receptor Quantification Service at Creative Biolabs
Amplified luminescent proximity homotransfer assay is a sensitive assay technology often used to quantify proteins, including their phosphorylated forms. When quantifying phosphorylated (activated) BTK versus total BTK, the assay can leverage specific antibodies that discriminate between the phosphorylated and non-phosphorylated forms of the protein. Creative Biolabs offers custom quantification services. These can be tailored to specific needs using different methodologies.
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Sample Preparation
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Tissue Samples: Fresh-frozen or paraffin-embedded tissues can be used.
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Cell Lines: Cultured cell lines expressing ErbB receptors.
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Body Fluids: Plasma or serum samples may also be analyzed.
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Quantification Methods
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Western Blotting: This technique identifies and quantifies the protein levels of ErbB receptors.
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Enzyme-Linked Immunosorbent Assay (ELISA): A high-throughput method suitable for quantifying soluble receptors or extracellular portions.
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Flow Cytometry: Useful for quantifying receptors on the surface of living cells.
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Immunohistochemistry (IHC): For visualizing and quantifying receptor localization in tissue samples.
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Real-Time PCR: To assess the expression levels of mRNA for these receptors.
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Analysis and Interpretation
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Data Analysis: Results may be normalized and quantified against controls or standards for accurate interpretation.
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Reporting: Detailed reports highlighting receptor levels, comparisons, and insights into the potential implications for clinical or research contexts.
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Consultation Services
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Experts at Creative Biolabs may provide guidance on experimental design, interpretation of results, and practical applications in the context of specific studies or therapeutic development.
Other Services
In addition to the above generic ErbB receptor quantification services, Creative Biolabs also provides the following methods and techniques for the assessment of ErbB receptor activation/inactivation:
Downstream Signaling Pathway Analysis
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The downstream signaling pathways activated by ErbB receptors include the PI3K/AKT, RAS/RAF/MEK/ERK, and PLCγ pathways.
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Luciferase Reporter Assays: Measure transcriptional activity resulting from pathway activation.
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Ligand Binding Assays
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Assess the binding affinity of ligands (e.g., EGF, NRG) to ErbB receptors, which can indicate receptor activation.
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Surface Plasmon Resonance (SPR): Measures real-time binding interactions.
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Radiolabeled Ligand Binding Assays: Use of radioactively labeled ligands to quantify binding.
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Frequently Asked Question
Q1: Applications of ErbB Receptor Quantification.
A1: The quantification of ErbB receptors is essential in several applications across medical and biotechnological fields:
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ErbB receptor quantification helps in identifying the subtype of cancer and its aggressiveness.
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Quantification helps in tailoring targeted therapies.
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Measuring receptor levels allows for an assessment of the effectiveness of targeted therapies over time.
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Understanding the role of ErbB receptors in signal transduction pathways can inform drug development and screening for new therapeutic agents targeting these receptors.
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Quantification is crucial in studies investigating the mechanisms of receptor activation, downstream signaling pathways, and cellular responses to various stimuli.
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Research on ErbB receptor expression can lead to the discovery of new biomarkers for various diseases, including inflammatory conditions and neurodegenerative diseases.
Contact Us
Thank you for your interest in our ErbB Receptor Quantification Service. At Creative Biolabs, we specialize in delivering precise and reliable quantification of ErbB receptors, vital for advancing research and therapeutic applications in oncology. If you have any questions, require further information, or wish to discuss your project requirements, please don't hesitate to reach out to us. We look forward to collaborating with you!
For Research Use Only | Not For Clinical Use
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