GIST-T1 In Vitro Calcein AM Cytotoxic Assay (Cell Viability)

CAT#: ITS-1122-YF2842
Target Cell Organism: Human
Target Cell Name: GIST-T1
Assay Type: Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Overview
This assay is to provide GIST-T1-based In Vitro Calcein AM Cytotoxic Assay (Cell Viability) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.
Target Cell Name
GIST-T1
Target Cell Organism
Human
Target Cell Background
A new human cell line, GIST-T1, was established from a metastatic plural tumor from a gastrointestinal stromal tumor (GIST) of the stomach in a Japanese woman, and was characterized by immunohistochemistry, conventional banding methods, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH).
Related Diseases
Gastrointestinal Stromal Tumor; GIST
Research Area
Oncology
Assay Name
In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Short Description
GIST-T1-cell based In Vitro Calcein AM Cytotoxic Assay (Cell Viability)
Assay Description
Calcein AM is another non-fluorescent cell permeable dye, which is converted to green-fluorescent calcein by viable intracellular esterases. Calcein violet AM, calcein red-orange, Oregon green 488 and 5-CFDA-AM are other examples of dyes used to monitor cell viability based on esterase activity in viable cells.
Assay Type
Cell Viability/Cytotoxicity and Antiproliferative Assays
Assay Type Details
Uncontrolled proliferation is one of the main features of cancer cells. Cell-based in vitro assays are employed to determine whether test molecules possess direct cytotoxic/antiproliferative effects in cancer cells.
Assay Subtype
Cell Viability Assay
Assay MOA
Cell-based Viability Assays Based on Cellular Enzymes and Proteins
For Research Use Only | Not For Clinical Use
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