GIST-T1 In Vitro RNA Expression Analysis Assay (Cap Analysis)

This assay is to provide GIST-T1-based In Vitro RNA Expression Analysis Assay (Cap Analysis) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

GIST-T1

Human

A new human cell line, GIST-T1, was established from a metastatic plural tumor from a gastrointestinal stromal tumor (GIST) of the stomach in a Japanese woman, and was characterized by immunohistochemistry, conventional banding methods, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH).

Gastrointestinal Stromal Tumor; GIST

Oncology

In Vitro RNA Expression Analysis Assay (Cap Analysis)

GIST-T1-cell based In Vitro RNA Expression Analysis Assay (Cap Analysis)

Cap analysis gene expression involves the sequencing of PCR-amplified cDNA tags obtained from the 5' end of mRNA. For the identification of starting sites of transcription, the cap analysis gene expression tech nique has become a useful platform.

Gene and Protein Expression Assays

Following transcription, primary transcript RNA is converted into mature RNA by post-transcriptional modifications. Post-transcriptional modification includes three major steps namely the addition of a 5'cap, addition of a 3'polyadenylation tail and RNA splicing. Stability of RNA has been considered as very important for gene expression and proteins at the post-transcriptional level.