GIST-T1 In Vitro Scratch Assay (Migration)

This assay is to provide GIST-T1-based In Vitro Scratch Assay (Migration) to accelerate our client's oncology projects. The assay will be customized according to the specific requirements. Please contact our scientists to discuss more details.

GIST-T1

Human

A new human cell line, GIST-T1, was established from a metastatic plural tumor from a gastrointestinal stromal tumor (GIST) of the stomach in a Japanese woman, and was characterized by immunohistochemistry, conventional banding methods, comparative genomic hybridization (CGH), and fluorescence in situ hybridization (FISH).

Gastrointestinal Stromal Tumor; GIST

Oncology

In Vitro Scratch Assay (Migration)

GIST-T1-cell based In Vitro Scratch Assay (Migration)

A wound healing assay is one of the simple and common assays used to monitor cancer cell migration. In a normal wound healing assay, attached cancer cells are exposed to tested compound (s) and incubated for 1-2 hours. A scratch is then manually made using a sterile pipette tip or needle and the width of the scratch is recorded at different time intervals. The effects of a testing drug on invasiveness of cells can also be monitored using this assay. However, this assay cannot be carried out with non-adherent cells.

Wound Healing Assay

Cell Migration and Invasion Assays

The movement of cancer cells and formation of tumors in neighboring tissue is a sequential cascade known as invasion-metastatic cascade, which includes cancer cell invasion through extracellular matrix (ECM), intravasation into blood and colonization at sites after they exit from blood circulation. Migration and invasion are two dif ferent processes where, cell migration involves movement of cells without passing through a barrier such as ECM, whereas cell invasion involves passing through a barrier such as ECM while destroying it.

Cell Migration Assay

Cell Migration Assay