Goat Endotoxin ELISA Kit (Competitive)-96 tests (CAT#: IO-HMM83)

96 tests

Microtiter plate; Enzyme conjugate; Standards; Substrate A; Substrate B; Stop solution; Wash solution; Balance solution

90 minutes

1)Add 50 µL of sample or standard to the appropriate wells in the supplied microtiter plate. 2)Incubate 1 hour at room temperature. 3)Empty wells and wash 3-5 times. 4)Empty final wash and add 100 µL of conjugate per well and mix well. Cover and incubate 1 hour at 37°C in a humid chamber. 5)Wash each well 5 times with wash solutions. 6)Add 50 µL substrate A to each well followed by addition of 50 µL substrate B. Cover and incubate 10-15 minutes at room temperature. 7)Add 50 µL substrate A to each well followed by addition of 50 µL substrate B. Cover and incubate 10-15 minutes at room temperature. 8)Immediately read the optical density (O.D.) at 450 nm. 9)Subtract the mean blank value from each sample or standard value and calculate the mean for duplicate (or greater) wells. 10)Construct the standard curve using graph paper or statistical software.

4°C