Short Description
Measures nitric oxide using a colorimetric method.
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Nitric Oxide Assay Kit offers a comprehensive colorimetric method for quantifying total nitric oxide production by measuring stable nitrate and nitrite metabolites. The system utilizes an enzymatic reduction step followed by the Griess reaction to produce a colored product. This reliable tool facilitates research into signaling pathways and vascular function, providing high sensitivity for fundamental studies in physiology, immunology, and metabolic regulation.
Features
Provides a robust two-step enzymatic method for measuring total nitric oxide production, supporting research into inflammatory signaling, vasodilation, and redox biology with high reproducibility.
Process Relevance
Monitoring nitric oxide metabolites to characterize signaling pathways and cellular response to stimuli.
Application Stage
Inflammation research and vascular signaling studies.
Applications
Quantify nitric oxide production in cell culture and biological fluids to study cardiovascular signaling and immune response mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 540nm
Sample Type
Serum, plasma, urine, saliva, cell culture supernatants.
Research Areas
Cardiovascular Research; Immunology; Neuroscience
Datasheet