GuardianOx™ Nitric Oxide Assay Kit

CAT#: ITS-1125-YL35
Product Type: Assay Kit
Species: All Species
Brand: GuardianOx
Development Stage: Research-Grade Oxidative Stress Assay Development
Short Description
Measures nitric oxide using a colorimetric method.
Brand
GuardianOx
Development Stage
Research-Grade Oxidative Stress Assay Development
Description
This GuardianOx™ Nitric Oxide Assay Kit offers a comprehensive colorimetric method for quantifying total nitric oxide production by measuring stable nitrate and nitrite metabolites. The system utilizes an enzymatic reduction step followed by the Griess reaction to produce a colored product. This reliable tool facilitates research into signaling pathways and vascular function, providing high sensitivity for fundamental studies in physiology, immunology, and metabolic regulation.
Features
Provides a robust two-step enzymatic method for measuring total nitric oxide production, supporting research into inflammatory signaling, vasodilation, and redox biology with high reproducibility.
Process Relevance
Monitoring nitric oxide metabolites to characterize signaling pathways and cellular response to stimuli.
Application Stage
Inflammation research and vascular signaling studies.
Applications
Quantify nitric oxide production in cell culture and biological fluids to study cardiovascular signaling and immune response mechanisms.
Qualified With
Internal performance validation using reference standards under defined assay conditions.
Detection Method
Absorbance at 540nm
Sample Type
Serum, plasma, urine, saliva, cell culture supernatants.
Research Areas
Cardiovascular Research; Immunology; Neuroscience
Size
100 tests
Data Output Type
Quantitative concentration measurement
Assay Time
40 min
LOD
LOD: 0.6 µM
Species
All Species
Storage
Store at -20 °C upon receipt to maintain reagent stability. Follow recommended handling procedures to ensure consistent assay performance.
Storage Comment
Avoid repeated freeze-thaw cycles. During use, minimize the time components are exposed to room temperature and return them promptly to -20 °C storage after handling. Do not store at elevated temperatures for extended periods.
Expiry Date
6 months under recommended storage conditions to ensure optimal performance.
Note
For bulk pricing or custom reagent inquiries, please contact us by email or phone. Products are shipped on ice via FedEx.
Restrictions
For Research Use Only (RUO). Not intended for diagnostic or therapeutic use.
Shipping
Shipped at room temperature.
Background
This gaseous signaling molecule is involved in a wide range of physiological processes, including vasodilation, neurotransmission, and immune response. Due to its short half-life, its activity is typically monitored by measuring its stable metabolites, nitrate and nitrite.
Pathways
TBARS are downstream products of the oxidative degradation of lipids initiated by free radical attack. They serve as indicators of impaired membrane integrity and the activation of lipid-mediated signaling cascades.
For Research Use Only | Not For Clinical Use
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